Vulnerable at extreme pH: most columns only provide results between pH 2 to 8. While it is true that silica-based columns work with most applications, they have several limitations which are: Anyhow, we should also take into account the separation mode and therefore, the selection of the functional group and/or analyte without forgetting the application area. The selection of a suitable HPLC column for a specific application is a crucial decision in order to obtain the best possible results regarding separation, resolution, costs, analysis time, etc.ĭuring this selection is important to take into account the base material/stationary phase (it is like the “foundation of a building”) which in the case of HPLC columns can be silica, polymer, etc.
